| Revista: | Brazilian Journal of Pharmaceutical Sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000451504 |
| ISSN: | 1984-8250 |
| Autors: | Requeijo, Thais Boscolo1 Serrano, Silvia Helena Pires2 Moya, Horacio Dorigan1 |
| Institucions: | 1Fundacao do ABC, Faculdade de Medicina, Santo Andre, Sao Paulo. Brasil 2Universidade de Sao Paulo, Instituto de Quimica, Sao Paulo. Brasil |
| Any: | 2021 |
| Volum: | 57 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Some antioxidant compounds have a pro-oxidant effect in the presence of transition metal ions, due to the reduction of Mn+ to M(n-1)+ with simultaneous formation of free radicals, which then promote DNA damage. In the present study, we evaluated the pUC19 DNA damage in a solution containing Cu(II) and ascorbic acid (AA) or S(IV) saturated with air by agarose gel electrophoresis. Our results showed that this damage decreases if AA and S(IV) are simultaneously added. This study also illustrates the importance of Cu(II) in this process, as no DNA damage was observed when AA or S(IV) were present in the absence of this metallic ion. Our data showed that DNA preservation depends on the concentration of AA and S(IV) and occurs when the [S(IV)]:[AA] ratio ranges from 1:1 to 20:1. Absorbance measurements and thermodynamic data show that no reaction occurs between AA and S(IV) when this mixture (pH 5.5) is added to pUC-19 DNA. The presence of dissolved oxygen may be the cause of AA consumption in the mixture of these two antioxidants, which subsequently decreases DNA damage |
| Disciplines | Química |
| Paraules clau: | Química farmacéutica, Antioxidantes, Daño al ADN, Cobre, Acido ascórbico, Sulfitos, Electroforesis en gel |
| Keyword: | Medicinal chemistry, Antioxidants, Gel electrophoresis, DNA damage, Copper, Ascorbic acid, Sulfites |
| Text complet: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
