| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308903 |
| ISSN: | 0104-6632 |
| Autors: | Rabelo, A.P.B1 Tambourgi, E.B |
| Institucions: | 1Universidade Estadual de Campinas, Faculdade de Engenharia Quimica, Campinas, Sao Paulo. Brasil |
| Any: | 2003 |
| Període: | Dic |
| Volum: | 20 |
| Número: | 4 |
| Paginació: | 357-362 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | This work presents the results obtained using a microcolumn agitated by pulsed caps, using aqueous two-phase systems formed polyethylene glycol and salts (monobasic and dibasic) potassium phosphate for protein extraction. Proteins used were extracted cytochrome b5 and the enzyme ascorbic oxidoreductase. It was observed that operation of this equipment was stable and high efficiency values were achieved |
| Disciplines | Química |
| Paraules clau: | Ingeniería química, Purificación de proteínas, Sistema acuoso bifásico, Procesamiento, Extracción continua, Extracción por solventes |
| Keyword: | Chemistry, Chemical engineering, Protein purification, Aqueous two-phase system, Downstream processing, Continuous extraction, Solvent extraction |
| Text complet: | Texto completo (Ver HTML) |
