| Revista: | Brazilian journal of chemical engineering |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000308967 |
| ISSN: | 0104-6632 |
| Autors: | Ferreira, L.S1 Trierweiler, J.O2 Souza-Junior, M.B. de Folly, R.O.M |
| Institucions: | 1Universidade Federal do Rio de Janeiro, Faculdade de Quimica, Rio de Janeiro. Brasil 2Universidade Federal do Rio Grande do Sul, Departamento de Engenharia Quimica, Porto Alegre, Rio Grande do Sul. Brasil |
| Any: | 2004 |
| Període: | Jun |
| Volum: | 21 |
| Número: | 2 |
| Paginació: | 307-315 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | In this work an enzymatic lactose biosensor composed of the immobilized enzymes b-galactosidase and glucose oxidase was developed. Oxygen consumption during the reaction catalyzed by these enzymes was detected. The biosensor was integrated into an FIA (flow injection analysis) system that allows measurement of lactose on-line in less than three minutes. This biosensor was used to monitor lactose concentration during the production of b-galactosidase by the yeast Kluyveromyces marxianus from cheese whey. The sensor showed good stability after four months and after almost 7000 measurements had been performed. The analytical curve was linear in the range of lactose concentration from 1 to 30 g/L |
| Disciplines | Química |
| Paraules clau: | Ingeniería química, Biotecnología, Lactosa, Biosensores, Análisis de inyección en flujo, Kluyveromyces marxianus |
| Keyword: | Chemistry, Chemical engineering, Biotechnology, Lactose, Biosensors, Flow injection analysis, Kluyveromyces marxianus |
| Text complet: | Texto completo (Ver HTML) |
