| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000372707 |
| ISSN: | 1516-8913 |
| Autors: | Jackisch-Matsuura, Ani Beatriz1 Santos, Leonardo Silva2 Eberlin, Marcos Nogueira3 Faria, Andreia Fonseca de6 Matsuura, Takeshi4 Grossman, Matthew James5 Durrant, Lucia Regina6 |
| Institucions: | 1Fundacao Instituto Oswaldo Cruz, Centro de Pesquisa Leonidas e Maria Deane, Manaus, Amazonas. Brasil 2Universidad de Talca, Instituto de Química de Recursos Naturales, Talca. Chile 3Universidade Estadual de Campinas, Instituto de Quimica, Campinas, Sao Paulo. Brasil 4Universidade Federal do Amazonas, Instituto de Ciencias Biologicas, Manaus, Amazonas. Brasil 5BioSage, Lawrenceville, New Jersey. Estados Unidos de América 6Universidade Estadual de Campinas, Faculdade de Ciências de Alimentos, Campinas, Sao Paulo. Brasil |
| Any: | 2014 |
| Període: | Ene-Feb |
| Volum: | 57 |
| Número: | 1 |
| Paginació: | 138-144 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and Rf 0.52 and 0.62, respectively. The partially purified SACs were characterized by the ESI(+)-MS, FT-IR and NMR. In each one of these fractions, a mixture of 10 oligomers was found consisting of a series of compounds, with masses from 502 to 899, differing in molecular mass by a repeating unit of 44 Daltons. The mass spectra of these compounds did not appear to match other known biosurfactants and could represent a novel class of these compounds |
| Disciplines | Biología |
| Paraules clau: | Bacterias, Ecología, Hongos, Bioemulsificantes, Biorremediación, Biosurfactantes, Química industrial, Producción, Surfactantes microbianos |
| Keyword: | Biology, Bacteria, Ecology, Fungi, Bioemulsifiers, Bioremediation, Biosurfactants, Gordonia amicalis, Industrial chemistry, Production, Microbial surfactants |
| Text complet: | Texto completo (Ver PDF) |
