Revista: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000372677 |
ISSN: | 1516-8913 |
Autors: | Singariya, Premlata1 Kumar, Padma1 Mourya, Krishan Kumar2 |
Institucions: | 1University of Rajasthan, Department of Botany, Jaipur. India 2Veterinary Hospital, Rajasthan. India |
Any: | 2014 |
Període: | Ene-Feb |
Volum: | 57 |
Número: | 1 |
Paginació: | 62-69 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental |
Resumen en inglés | The present study was carried out to determine the possible bioactive components (steroids) of Cenchrus setigerus using GC-MS analysis and in vivo estimation of metabolites (total soluble sugar, soluble protein, proline and total phenolics), photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) of seedlings and antimicrobial activity of extracts in various polar solvents from the leaves of C. setigerus. Antimicrobial activity was evaluated against three Gram-negative bacteria, including Proteusmirabilis, Klebsiella pneumonia and Agrobacterium tumefaciens andone fungus Aspergillus niger using 'disc diffusion' method, followed by the determination of minimum inhibitory concentrations (MIC) by broth dilution method. Results revealed the presence of some steroids in the isopropyl alcohol extract of C. setigerus:which are (22E)-stigmasta-4,22-dien-3-one(4.93%), δ4-sitosterol-3-one (stigmast-4-en-3-one) (4.31%), fagarsterol (lupeol) (1.25%) and ethyl iso-allocholate (0.32%). Total soluble sugars and chlorophyll-a were also recorded to be highest. The highest activity was exhibited by the isopropyl alcohol and ethyl acetate extract against P. mirabilis |
Disciplines | Agrociencias, Química |
Paraules clau: | Gramíneas, Fitoquímica, Química farmacéutica, Cenchrus setigerus, Pigmentos fotosintéticos, Prolina, Antibióticos, Determinación MIC |
Keyword: | Agricultural sciences, Chemistry, Gramineae, Medicinal chemistry, Phytochemistry, Cenchrus setigerus, Photosynthetic pigments, Total soluble sugar, Proline, Antibacterial activity, MIC determination, MBC/MFC |
Text complet: | Texto completo (Ver PDF) |