In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia



Título del documento: In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000372622
ISSN: 1516-8913
Autors: 1
1
1
1
Institucions: 1Twintech International University, Faculty of Biotechnology, Kuala, Lumpur. Malasia
Any:
Període: Ene-Feb
Volum: 57
Número: 1
Paginació: 23-28
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP) and Kinetin (Kn) for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and 1-Naphthaleneacetic acid (NAA) for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants) was observed on a Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants) was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in plant soil with survival rate of 83.3 %
Disciplines Agrociencias,
Biología,
Química
Paraules clau: Fitotecnia,
Fisiología vegetal,
Química farmacéutica,
Stevia rebaudiana,
Medio MS,
Edulcorantes,
Proliferación
Keyword: Agricultural sciences,
Biology,
Chemistry,
Crop husbandry,
Plant physiology,
Medicinal chemistry,
Stevia rebaudiana,
MS medium,
Sweetening agents,
Proliferation
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