| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000373453 |
| ISSN: | 1516-8913 |
| Autors: | Santos-Filho, Plinio Rodrigues dos1 Santos, Breno Regis2 Barbosa, Sandro2 Vieira, Letícia Rios2 Freitas, Natalia Chagas de2 Dias, Daniele Ferreira3 Santos, Marcelo Henrique dos4 |
| Institucions: | 1Universidade Federal de Alfenas, Instituto de Ciencias Biomedicas, Alfenas, Minas Gerais. Brasil 2Universidade Federal de Alfenas, Instituto de Ciencias da Natureza, Alfenas, Minas Gerais. Brasil 3Universidade Federal de Alfenas, Instituto de Quimica, Alfenas, Minas Gerais. Brasil 4Universidade Federal de Vicosa, Departamento de Quimica, Vicosa, Minas Gerais. Brasil |
| Any: | 2014 |
| Període: | May-Jun |
| Volum: | 57 |
| Número: | 3 |
| Paginació: | 326-333 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Garcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine for its therapeutic properties. This plant is rich in bioactive compounds like benzophenones. However, there are no works about the in vitro establishment and achievement of secondary metabolites in this plant. Thus, the aim of this work was to determine the growth curve and to perform the biochemical and phytochemical analyses in calli obtained from the procambium segments of Bacupari. The growth curve of calli followed a sigmoidal pattern, with four distinct phases (lag, exponential, linear, deceleration). Total soluble sugars were higher on the inoculation day and the reducing sugars on the 20 th day. Amino acids increased from the 60 th day up to the stabilization on the 120 th day. The protein content varied, but it seemed to be related to the amino acids metabolism. The phytochemical screening showed the presence of phenolic and flavonoid compounds in the calli and the HPLC analysis allowed the identification of Fukugetin, Guttiferone A and 7-epiclusianone |
| Disciplines | Biología, Química |
| Paraules clau: | Angiospermas, Fisiología vegetal, Fitoquímica, Plantas medicinales, Cultivo de tejidos, Curvas de crecimiento, Benzofenona, Garcinia brasiliensis, Clusiaceae |
| Keyword: | Biology, Chemistry, Angiosperms, Plant physiology, Phytochemistry, Medicinal plants, Tissue culture, Growth curves, Benzophenone, Garcinia brasiliensis, Clusiaceae |
| Text complet: | Texto completo (Ver HTML) |
