| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000374044 |
| ISSN: | 1516-8913 |
| Autors: | Zia, Muhammad Anjum1 Riaz, Ayesha1 Rasul, Samreen1 Abbas, Rao Zahid2 |
| Institucions: | 1University of Agriculture, Department of Chemistry and Biochemistry, Faisalabad. Pakistán 2University of Agriculture, Department of Parasitology, Faisalabad. Pakistán |
| Any: | 2013 |
| Període: | Nov-Dic |
| Volum: | 56 |
| Número: | 6 |
| Paginació: | 956-961 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion method against selected microbial strains where glucose oxidase from A. niger showed anti-bacterial activity, while no fungicidal effects were shown by both A. niger and P. notatum glucose oxidases |
| Disciplines | Biología, Química |
| Paraules clau: | Hongos, Microbiología, Farmacología, Actividad antimicrobiana, Glucosa oxidasa, Aspergillus niger, Penicillium notatum |
| Keyword: | Biology, Chemistry, Fungi, Microbiology, Pharmacology, Antimicrobial activity, Glucose oxidase, Aspergillus niger, Penicillium notatum |
| Text complet: | Texto completo (Ver HTML) |
