Enhanced immune response of a bicistronic DNA vaccine expressing fusion antigen Hsp65-Esat-6 of Mycobacterium tuberculosis with GM-CSF as a molecular adjuvant
Título del documento: Enhanced immune response of a bicistronic DNA vaccine expressing fusion antigen Hsp65-Esat-6 of Mycobacterium tuberculosis with GM-CSF as a molecular adjuvant
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000366253
ISSN: 1516-8913
Autors: 1
1
1
1
Institucions: 1Jinan University, College of Life Science and Technology, Guangzhou, Guangdong. China
Any:
Període: Sep-Oct
Volum: 56
Número: 5
Paginació: 757-765
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés This study aimed to construct a bicistronic DNA vaccine expressing fusion antigen Hsp65-Esat-6 of Mycobacterium tuberculosis with cytokine GM-CSF as a molecular adjuvant (pIRES-Hsp65-ESAT-6-GM-CSF, pIRHEG), and the immune response in mice. C57BL/6 mice were immunized with the recombinant plasmid to detect the titer of antibodies, lymphocyte proliferation, the ratio of CD4+, CD8+T cell and IFN ~ γ，IL-2 secretion. The titer of antibody, lymphocyte proliferation, the ratio of CD4+T and CD8+T cells and IFN ~ γ, IL-2 secretion of pIRHEG group was significant higher than other recombinant plasmid groups, which significant differed by statistical mean. The bicistronic DNA vaccine could induce an effective immune response in mice and could be used as vital ingredient of a new tuberculosis vaccine candidate
Disciplines Biología,
Agrociencias,
Medicina
Paraules clau: Inmunología,
Parasitología,
Vacunas,
ADN,
Antígenos,
Mycobacterium tuberculosis,
Adyuvantes
Keyword: Biology,
Agricultural sciences,
Medicine,
Immunology,
Parasitology,
Vaccines,
DNA,
Antigens,
Mycobacterium tuberculosis,
Adjuvants
Text complet: Texto completo (Ver HTML)