| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000392691 |
| ISSN: | 1516-8913 |
| Autors: | Elsayed, Elsayed Ahmed1 Omar, Hoda Gamal2 El-Enshasy, Hesham Ali3 |
| Institucions: | 1King Saud University, Faculty of Science, Riyadh. Arabia Saudita 2National Research Center, Natural and Microbial Products Department, Dokki, Cairo. Egipto 3Alexandria University, Faculty of Pharmacy, Alejandría. Egipto |
| Any: | 2015 |
| Període: | Sep-Oct |
| Volum: | 58 |
| Número: | 5 |
| Paginació: | 676-685 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Oxytetracycline (OTC) production by Streptomyces rimosus was studied in batch and fed - batch cultures in shake flask and bioreactor levels using semi - defined medium. First, the effect of glucose concentration on OTC production and growth kinetics was studied intensively. The optimal glucose concentration in the medium was 15 g/L. Higher glucose concentrations supported higher biomass production by less volumetric and specific antibiotic production. Based on these data, cultivations were carried out at semi - industrial scale 15 L bioreactor in batch culture. At bioreactor level, cell growth and OTC production were higher compared to the shake flask culture by about 18 and 38%, respectively. During the bioreactor cultivation, glucose was totally consumed after only 48 h. Thus, the fed - batch experiment was designed for mono - glucose feeding and complete medium feeding to increase the OTC production by overcoming carbon limitations. The results showed that the fed - batch culture using constant glucose feeding strategy with rate of 0.33 g/L/h pro duced 1072 mg/L. On the other hand, feeding with complete medium resulted in 45% higher biomass but less OTC production by about 26% compared to mono - glucose fed culture. A further improvement in this process was achieved in by keeping the dissolved oxygen (DO) value at 60% saturation by cascading the glucose feeding pump with the DO controller. The later feeding strategy resulted in higher antibiotic production, reaching 1414 mg/L after 108 h |
| Disciplines | Biología, Química |
| Paraules clau: | Bacterias, Química farmacéutica, Oxitetraciclina, Streptomyces rimosus, Biorreactores, Cultivo, Antibióticos, Biotecnología |
| Keyword: | Biology, Chemistry, Bacteria, Medicinal chemistry, Oxytetracycline, Streptomyces rimosus, Bioreactors, Culture, Antibiotics, Biotechnology |
| Text complet: | Texto completo (Ver PDF) |
