| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000387701 |
| ISSN: | 1516-8913 |
| Autors: | Souza, Carlos Eduardo Alves de1 Stolf, Aline Maria1 Dreifuss, Arturo Alejandro1 Lívero, Francislaine dos Reis1 Gomes, Liana de Oliveira;1 Petiz, Lyvia2 Beltrame, Olair3 Dittrich, Rosangela Locatelli3 Acco, Alexandra1 Telles, Jose Ederaldo Queiroz4 Cadena, Sílvia Maria2 |
| Institucions: | 1Universidade Federal do Parana, Departamento de Farmacologia, Curitiba, Parana. Brasil 2Universidade Federal do Parana, Departamento de Bioquimica e Biologia Molecular, Curitiba, Parana. Brasil 3Universidade Federal do Parana, Departamento de Medicina Veterinaria, Curitiba, Parana. Brasil 4Universidade Federal do Parana, Departamento de Patologia Medica, Curitiba, Parana. Brasil |
| Any: | 2015 |
| Període: | May-Jun |
| Volum: | 58 |
| Número: | 3 |
| Paginació: | 367-378 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Alcoholic liver disease is characterized by a wide spectrum of liver damage, which increases when ethanol is associated with high-fat diets (HFD). This work aimed to establish a model of alcoholic hepatic steatosis (AHS) by using a combination of 10% ethanol and sunflower seeds as the source of HFD. Male rats received water or 10% ethanol and regular chow diet and/or HFD, which consisted of sunflower seeds. The food consumption, liquid intake and body weight of the rats were monitored for 30 days. After this period, blood was collected for biochemical evaluation, and liver samples were collected for histological, mitochondrial enzyme activity and oxidative stress analyses. Our results indicated that the combination of 10% ethanol and HFD induced micro- and macrosteatosis and hepatocyte tumefaction, decreased the levels of reduced glutathione and glutathione S-transferase activity and increased the level of lipoperoxidation and superoxide dismutase activity. The mitochondrial oxidation of NADH and succinate were partially inhibited. Complexes I and II were the main inhibition sites. Hepatic steatosis was successfully induced after 4 weeks of the diet, and the liver function was modified. The combination of 10% ethanol and sunflower seeds as an HFD produced an inexpensive model to study AHS in rats |
| Disciplines | Biología, Medicina |
| Paraules clau: | Gastroenterología, Medicina experimental, Esteatosis, Hígado, Etanol, Dieta hiperlipídica, Mitocondrias, Modelos patológicos |
| Keyword: | Biology, Medicine, Experimental medicine, Gastroenterology, Steatosis, Liver, Ethanol, Hyperlipidic diet, Mitochondria, Pathological models |
| Text complet: | Texto completo (Ver HTML) |
