| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000330051 |
| ISSN: | 1516-8913 |
| Autors: | Wang, Tao1 Jiang, An -li2 Zhang, Jian-ye2 Jing, Fei1 |
| Institucions: | 1Taishan Medical University, Institution of Biochemistry, Taian City, Shandong. China 2Medical School of Shandong University, Institution of Biochemistry and Molecular Biology, Jinan, Shandong. China |
| Any: | 2010 |
| Període: | Mar-Abr |
| Volum: | 53 |
| Número: | 2 |
| Paginació: | 327-334 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated specific binding of the E2F decoy to E2F transcription factor.The PC-3M cell growth was inhibited after transfection. The apoptotic rate was 26.35% and DNA ladder could be observed after transfection.The expression of C-myc and CyclinD1 were inhibited. All these results indicated that E2F decoy DNA induced apoptosis of androgen-independent prostate cancer cell lines PC-3M and inhibited cell proliferation via inhibiting expression of C-myc and CyclinD1 |
| Disciplines | Medicina |
| Paraules clau: | Medicina experimental, Oncología, Cáncer, Próstata, Líneas celulares, Apoptosis, Factores de transcripción |
| Keyword: | Medicine, Experimental medicine, Oncology, Cancer, Prostate, Cell lines, Apoptosis, Transcription factors |
| Text complet: | Texto completo (Ver HTML) |
