| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000372676 |
| ISSN: | 1516-8913 |
| Autors: | Gowda, Vaishnavi1 Shivakumar, Srividya1 |
| Institucions: | 1Jain University, Center for PG Studies, Bangalore, Karnataka. India |
| Any: | 2014 |
| Període: | Ene-Feb |
| Volum: | 57 |
| Número: | 1 |
| Paginació: | 55-61 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | The study identified the innate enzymatic potential (amylase) of the PHB producing strain B. thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme). Comparative polyhydroxyalkanoate (PHA) production by B. thuringiensis IAM 12077 in biphasic growth conditions using glucose and starch showed appreciable levels of growth (5.7 and 6.8 g/L) and PHA production (58.5 and 41.5%) with a PHA yield of 3.3 and 2.8 g/L, respectively. Nitrogen deficiency supported maximum PHA yield (2.46 g/L) and accumulation (53.3%). Maximum growth (3.6 g/L), PHB yield (2.6 g/L) and PHA accumulation (72.8%) was obtained with C:N ratio of 8:1 using starch as the carbon source (10 g/L). Nine substrates (agro and food wastes) viz. rice husk, wheat bran, ragi husk, jowar husk, jackfruit seed powder, mango peel, potato peel, bagasse and straw were subjected to two treatments- acid hydrolysis and hydrolysis by innate enzymes, and the reducing sugars released thereby were utilized for polymer production. All the substrates tested supported comparable PHB production with acid hydrolysis (0.96 g/L-8.03 g/L) and enzyme hydrolysis (0.96 g/L -5.16 g/L). Mango peel yielded the highest PHB (4.03 g/L; 51.3%), followed by jackfruit seed powder (3.93 g/L; 29.32%). Varied levels of amylase activity (0.25U-10U) in all the substrates suggested the enzymatic hydrolysis of agrowastes |
| Disciplines | Biología, Química |
| Paraules clau: | Bacterias, Bioquímica, Química de polímeros, Bacillus thuringiensis, Polihidroxibutirato, Enzimas |
| Keyword: | Biology, Chemistry, Bacteria, Biochemistry, Polymer chemistry, Bacillus thuringiensis, Polyhydroxybutyrate, Hydrolytic enzymes, Enzymes |
| Text complet: | Texto completo (Ver PDF) |
