| Revista: | Botanical sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000380644 |
| ISSN: | 2007-4298 |
| Autors: | Choi, Jae-Suk1 Lee, Kyungyong2 Lee, Bo-Bae1 Kim, Yang-Chun2 Kim, Young Dae3 Hong, Yong-Ki4 Cho, Kwang Keun5 Choi, In Soon6 |
| Institucions: | 1Silla University, Industry-Academic Cooperation Foundation, Busan. Corea del Sur 2Gijang Local Products Co. Ltd., Busan. Corea del Sur 3National Fisheries Research & Development Institute, East Sea Fisheries Research Institute, Gangneung. Corea del Sur 4Pukyong National University, Department of Biotechnology, Busan. Corea del Sur 5Gyeongnam National University of Science and Technology, Department of Animal Resources Technology, Jinju. Corea del Sur 6Silla University, Department of Biological Science, Busan. Corea del Sur |
| Any: | 2014 |
| Període: | Sep |
| Volum: | 92 |
| Número: | 3 |
| Paginació: | 425-431 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Methanol extract of the marine brown alga Ecklonia cava has been shown to exhibit antimicrobial activity against Propionibacterium acnes. To identify the compounds conferring this antimicrobial activity, methanol extract was fractionated using silica-gel column chromatography by increasing solvent polarity, and the antimicrobial activities of each fraction were determined in terms of the minimum inhibitory concentration. Hexane, diethyl ether, acetone, ethyl acetate, acetonitrile, methanol, and water fractions were generated, with the acetone fraction exhibiting the greatest antibacterial activity (minimum inhibitory concentration = 156 μg/mL). The acetone fraction was further purified by Sephadex LH-20 column chromatography and reverse-phase HPLC using an Alltima C18 column with an H2O-MeOH linear gradient from 30 to 100%. Two peaks were eluted at 18.1 (P1) and 23.8 (P2) min, both of which exhibited marked antibacterial activity (minimum inhibitory concentration = 39 μg/mL for both). The structures of these compounds were determined on the basis of 1H and 13C nuclear magnetic resonance data and, through comparison with published data, can be phloroglucinol derivatives dieckol (P1) and phlorofucofuroeckol-A (P2). These results suggest that phlorotannins derived from E. cava have antimicrobial activity against P. acnes, and so may be useful as natural additives in anti-acne cosmetics and pharmaceutical products |
| Disciplines | Biología, Química |
| Paraules clau: | Algas, Bacterias, Fitoquímica, Ecklonia cava, Propionibacterium acnes, Algas pardas, Algas marinas, Actividad antibacteriana, Florotaninos, Florofucofuroecol-A, Diecol, Aditivos naturales, Concentración mínima inhibitoria |
| Keyword: | Biology, Chemistry, Algae, Bacteria, Phytochemistry, Ecklonia cava, Propionibacterium acnes, Antibacterial activity, Dieckol, phlorotannins, Phlorofucofuroeckol-A, Minimum inhibitory concentration, Natural additives |
| Text complet: | Texto completo (Ver HTML) |
