| Revista: | Biocell |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000321421 |
| ISSN: | 0327-9545 |
| Autors: | Joubert, Annie1 Marais, Sumari1 Maritz, Christine2 |
| Institucions: | 1University of Pretoria, Department of Physiology, Pretoria, Transvaal. Sudáfrica 2University of Pretoria, Department of Biochemistry, Pretoria, Transvaal. Sudáfrica |
| Any: | 2009 |
| Període: | Abr |
| Volum: | 33 |
| Número: | 1 |
| Paginació: | 67-70 |
| País: | Argentina |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these proteins may be time- and cell type dependent and thus not evident during the mitotic block observed. Investigation of the regulation of the B-cell lymphoma 2 family will allow researchers to consider signaling pathways for diseases where apoptosis can potentially be controlled |
| Disciplines | Medicina, Biología |
| Paraules clau: | Farmacología, Oncología, Biología celular, Carcinoma, Mama, Apoptosis, 2-Metoxiestradiol, Expresión proteica |
| Keyword: | Medicine, Biology, Oncology, Pharmacology, Cell biology, Carcinoma, Breast, Apoptosis, 2-methoxyestradiol, Protein expression |
| Text complet: | Texto completo (Ver PDF) |
