| Revista: | Biocell |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000320486 |
| ISSN: | 0327-9545 |
| Autors: | Lima, Eleonidas Moura1 Leal, Mariana Ferreira3 Smith, Marilia de Arruda Cardoso3 Burbano, Rommel Rodriguez4 Assumpcao, Paulo Pimentel de5 Bello, Maria Jose6 Rey, Juan Antonio6 Lima, Francinaldo Ferreira de7 Casartelli, Cacilda2 |
| Institucions: | 1Universidade Federal do Piaui, Colegiado de Biomedicina, Parnaiba, Piaui. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil 3Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo. Brasil 4Universidade Federal do Para, Instituto de Ciencias Biologicas, Belem, Para. Brasil 5Hospital Joao de Barros Barreto, Servico de Cirurgia, Belem, Para. Brasil 6Instituto de Investigaciones Biomédicas, Madrid. España 7Universidade Federal do Para, Colegiado de Ciencias Biologicas, Altamira, Para. Brasil |
| Any: | 2008 |
| Període: | Dic |
| Volum: | 32 |
| Número: | 3 |
| Paginació: | 237-243 |
| País: | Argentina |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric cancer samples. MLH1 and PMS2 methylation were associated with neoplastic samples compared to nonneoplastic ones. PMS2 methylation was associated with diffuse-and intestinal-type cancer compared with normal controls. Intestinal-type cancer showed significant association with MLH1 methylation. Diffuse-type cancer was significantly associated with MSH2 methylation. Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that methylation is associated with gastric carcinogenesis. Methylation of mismatch repair genes was associated with gastric carcinogenesis and may be a helpful tool for diagnosis, prognosis and therapies. However, MSH6 does not seem to be regulated by methylation in our samples |
| Disciplines | Medicina |
| Paraules clau: | Gastroenterología, Oncología, Genética, Reparación genética, Metilación, Cáncer gástrico |
| Keyword: | Medicine, Gastroenterology, Oncology, Genetic repairment, Methylation, Gastric cancer, Genetics |
| Text complet: | Texto completo (Ver HTML) |
