Cytogenetical and ultrastructural effects of copper on root meristem cells of Allium sativum L



Título del documento: Cytogenetical and ultrastructural effects of copper on root meristem cells of Allium sativum L
Revista: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000321415
ISSN: 0327-9545
Autors: 1
2
1
1
1
1
Institucions: 1Tianjin Normal University, Department of Biology, Tianjin. China
2Tianjin Normal University, Library, Tianjin. China
Any:
Període: Abr
Volum: 33
Número: 1
Paginació: 25-32
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Different copper concentrations, as well as different exposure times, were applied to investigate both cytogenetical and ultrastructural alterations in garlic (Allium sativum L.) meristem cells. Results showed that the mitotic index decreased progressively when either copper concentration or exposure time increased. C-mitosis, anaphase bridges, chromosome stickiness and broken nuclei were observed in the copper treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. Also, increased dictyosome vesicles in number, formation of cytoplasmic vesicles containing electron dense granules, altered mitochondrial shape, disruption of nuclear membranes, condensation of chromatin material, disintegration of organelles were observed. The mechanisms of detoxification and tolerance of copper are briefly discussed
Disciplines Medicina,
Biología
Paraules clau: Toxicología,
Angiospermas,
Cobre,
Citotoxicidad,
Análisis citogenético,
Mitosis,
Ajo,
Allium sativum
Keyword: Medicine,
Biology,
Toxicology,
Angiosperms,
Copper,
Cytotoxicity,
Cytogenetic analysis,
Mitosis,
Garlic,
Allium sativum
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