Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro
Título del documento: Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro
Revista: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000320487
ISSN: 0327-9545
Autors: 1
2
2
2
2
Institucions: 1Yonsei University, Brain Korea 21 Project for Medical Science, Seúl. Corea del Sur
2Yonsei University College of Medicine, Institute of Vision Research, Seúl. Corea del Sur
Any:
Període: Dic
Volum: 32
Número: 3
Paginació: 245-250
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick end-labeling (TUNEL) method. As a result, differentiated RGC-5 cells were found to have decreased viability after addition of hydrogen peroxide in a dose-dependent manner. This hydrogen peroxide induced cytotoxicity caused apoptosis characterized by DNA fragmentation. Agmatine pretreatment not only increased cell viability but also attenuated DNA fragmentation. In conclusion, agmatine pretreatment demonstrated neuroprotective effects against oxidative stress induced by hydrogen peroxide in differentiated RGC-5 cells in vitro. This suggests a novel therapeutic strategy rescuing retinal ganglion cells from death caused by oxidative injury
Disciplines Biología,
Medicina,
Química
Paraules clau: Biología celular,
Neurología,
Bioquímica,
Neuroprotección,
Apoptosis,
Estrés oxidativo,
Daño neuronal,
Células ganglionares retinianas
Keyword: Biology,
Medicine,
Chemistry,
Cell biology,
Neurology,
Biochemistry,
Neuroprotection,
Apoptosis,
Oxidative stress,
Neuronal damage,
Retinal ganglion cells
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