Dynamic changes of pro-inflammatory cytokines, adhesion molecules and lymphocytes activation markers as early indicators of diseases severity in patients with Dengue



Título del documento: Dynamic changes of pro-inflammatory cytokines, adhesion molecules and lymphocytes activation markers as early indicators of diseases severity in patients with Dengue
Revista: Avances en biomedicina
Base de datos: PERIÓDICA
Número de sistema: 000376280
ISSN: 2244-7881
Autors: 1
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Institucions: 1Universidad de Los Andes, Escuela de Medicina, Mérida. Venezuela
2Laboratorio Regional de Diagnóstico e Investigación del Dengue y Otras Enfermedades Virales, Aragua, Carabobo. Venezuela
Any:
Període: May-Ago
Volum: 3
Número: 2
Paginació: 65-75
País: Venezuela
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en español Varios mecanismos inmuno-patogénicos se han propuesto para explicar el incremento masivo de la permeabilidad vascular observada en las formas severas de la infección por el Virus del Dengue (DENV). El objetivo del estudio fue determinar los cambios cinéticos de mediadores inflamatorios (IL-8, TNF-α), marcadores soluble de activación linfocítica temprana (sIL-2R, sTNF-Rp75) y fracciones solubles de moléculas de adhesión celular (sICAM-1, sVCAM-1) como marcadores tempranos de severidad en pacientes con dengue. Veinte pacientes clasificados como Dengue (Dengue±Signos de Alarma,D±WS) y treinta pacientes con Dengue Severo (DS) fueron incluidos en el estudio. Suero de individuos aparentemente saludables fueron incluidos como controles. En comparación con los individuos controles, los casos con Dengue mostraron niveles de IL-8 y TNF con diferencias no significativas en la fase febril o crítica de la enfermedad; sin embargo, un incremento significativo de sICAM-1 y sVCAM-1 ocurrió en ambas fases, mientras que los niveles de sIL2R y sTNF-p75 se elevaron significativamente solo en la fase crítica de la enfermedad. En comparación con los casos con dengue y controles, los pacientes con DS
Resumen en inglés Several immunopathogenic mechanisms have been proposed to explain the massive increase of vascular permeability observed in the severe forms of infection by Dengue Virus (DENV). Our aim was to determine the kinetic changes of inflammatory mediators (IL-8, TNF- α), soluble early lymphocyte activation markers (sIL-2R, sTNF-Rp75) and soluble fractions of cell adhesion molecules (sICAM-1 and sVCAM-1) as indicators for early recognition of disease severity in patients with laboratory-confirmed dengue. Twenty patients classified as Dengue±Warning Signs (D±WS) and thirty patients with Severe Dengue (SD) were included in the study. Serums of apparently healthy individuals were included as controls. Compared with normal subjects, D±WS cases did not show significant differences in the levels of IL-8 or TNF-α during the acute nor in the critical stages of the disease; however, in D±WS cases levels of sICAM-1 and sVCAM-1 were higher than controls during both phases; in contrast, significant increase of sTNF-p75 and sIL2R levels were observed during the critical phase of the disease. Compared with both dengue patients and controls, patients with SD showed significant rise in the levels of IL-8 and TNF-α during the critical phase of the disease and a significant increase in adhesion molecules were detected in both phases, but the highest levels of sVCAM-1 and sIL-2R were observed only during the acute stage of the disease. In conclusion, sIL-2R and sVCAM-1, as early markers of lymphocyte and endothelial activation, would serves as indicators of severity during the acute phase of dengue infection
Disciplines Medicina
Paraules clau: Microbiología,
Inmunología,
Dengue,
Respuesta inmune,
Citocinas,
Daño endotelial,
Moléculas de adhesión,
Linfocitos
Keyword: Medicine,
Microbiology,
Immunology,
Dengue,
Immune response,
Cytokines,
Endothelial damage,
Adhesion molecules,
Lymphocytes
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