| Revista: | ARBS. Annual review of biomedical sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000351097 |
| ISSN: | 1678-4618 |
| Autors: | Nagata, Tetsuji1 |
| Institucions: | 1Shinshu University School of Medicine, Department of Anatomy and Cell Biology, Matsumoto. Japón |
| Any: | 2010 |
| Volum: | 12 |
| Paginació: | 1-29 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Study aging changes of intramitochondrial DNA, RNA, protein synthesis of mouse organs during the development and aging, 30 groups of developing and aging mice (3 individuals each), from fetal day 19 to postnatal newborn at day 1, 3, 9, 14 and adult at month 1, 2, 6, 12 to 24, were injected with either 3H-thymidine, 3H-uriidine, or 3H-leucine, sacrificed 1 h later and liver, adrenal, lung and testis tissues observed by electron microscopic radioautography. Accordinlgy, numbers of mitochondria per cell profile area, numbers of labeled mitochondria and the mitochondrial labeling index labeled with 3H-labeled precursors showing DNA, RNA, protein synthesis in these cells (hepatocytes, 3 zones of the adrenal cortices - zona glomerulosa, fasciculata and reticularis -, adrenal medullary cells, pulmonary cells and testis cells) were counted per cells and compared among the respective developing and aging groups. The numbers of mitochondria in these cells increased from fetal day 19 to postnatal month 1 and 2. However, the numbers of labeled mitochondria and the labeling indices of intramitochondrial DNA, RNA, protein syntheses incorporating the 3H-labeled precursors in the described tissue cells increased from fetal day 19 to postnatal month 1 and decreased to month 24. These data support that the activity of intramitochnodrial DNA, RNA, protein syntheses in cells of these tissues increased and decreased by development and aging in mice. The intramitochondrial DNA, RNA and protein syntheses in some other organs were also reviewed and discussed |
| Disciplines | Biología |
| Paraules clau: | Biología celular, Bioquímica, Síntesis proteica, Envejecimiento celular, Mitocondria, Radioautografía |
| Keyword: | Biology, Cell biology, Biochemistry, Protein synthesis, Cell aging, Mitochondria, Radioautography |
| Text complet: | Texto completo (Ver PDF) |
