| Revista: | Annals of hepatology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000407482 |
| ISSN: | 1665-2681 |
| Autors: | Sribenja, Sirinapa1 Natthasirikul, Nichapavee2 Vaeteewoottacharn, Kulthida1 Sawanyawisuth, Kanlayanee1 Wongkham, Chaisiri1 Jearanaikoon, Patcharee3 Wongkham, Sopit1 |
| Institucions: | 1Khon Kaen University, Faculty of Medicine, Khon Kaen. Tailandia 2Khon Kaen University, Faculty of Associated Medical Sciences, Khon Kaen. Tailandia |
| Any: | 2016 |
| Període: | Jul-Ago |
| Volum: | 15 |
| Número: | 4 |
| Paginació: | 577-585 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | 5-Fluorouracil (5-FU) is the most commonly used chemotherapeutic drug in the treatment of cholangiocarcinoma (CCA). Since development of drug resistance to 5-FU in CCA patients is the primary cause of treatment failure, a better understanding of the mechanism of drug resistance of this cancer is essential to improve the efficacy of 5-FU in CCA therapy. Material and methods. Material and methods. A Material and methods. 5-FU resistant CCA cell line (M214-5FUR) for a comparative chemo-resistance study was established. Real time RT-PCR was used to determine gene expression levels. Cell cytotoxicity was measured by the MTT assay. Protein expression levels were detected by the immunofluorescene method. Results. Results. It Results. was found that 5-FU resistance was associated with the overexpression of Tβ10 in CCA cell lines. 5-FU treatment at various concentrations induced the expressions of Tβ10 and ABC transporters (ABCB1, ABCG2 ABCA3) in two CCA cell lines, KKU-M055 and KKU-M214. M214-5FUR, a 5-FU-resistant cell line, exhibited a 5-FU resistant phenotype with a 16-fold extremely high expression of Tβ10 and ABC transporters, as compared to the parental cells, KKU-M214. siRNA targeted to Tβ10 significantly reduced expression of ABC transporters tested in the M214-5FUR cells (P < 0.05). Conclusions. Conclusions. The Conclusions. present novel findingsof Tβ10 connected with drug resistance as shown in this study provides a new insight for the therapeutic value of Tβ10 as a predictive biomarker of 5-FU chemoresistance. Inhibiting Tβ10 may be a valuable adjunct for suppression of ABC transporters and sensitizing chemotherapy treatment, especially 5-FU in CCA patients |
| Disciplines | Medicina |
| Paraules clau: | Gastroenterología, Oncología, Terapéutica y rehabilitación, Colangiocarcinoma, Quimioterapia, 5-fluorouracilo, Marcadores biológicos, Timosina |
| Keyword: | Medicine, Gastroenterology, Oncology, Therapeutics and rehabilitation, Cholangiocarcinoma, Chemotherapy, 5-fluoruracil, Biological markers, Thymosin |
| Text complet: | Texto completo (Ver PDF) |
