| Revista: | Annals of hepatology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000418121 |
| ISSN: | 1665-2681 |
| Autors: | Yi-Sun Song1 Cheng-Hu Fang2 Byung-Im So1 Jun-Young Park1 Dae Won Jun3 Kyung-Soo Kim1 |
| Institucions: | 1Hanyang University, Graduate School of Biomedical Science and Engineering, Seúl. Corea del Sur 2Yanbian University, Division of Cardiology, Yantái, Shandong. China 3Hanyang University, College of Medicine, Seúl. Corea del Sur |
| Any: | 2013 |
| Període: | Feb |
| Volum: | 12 |
| Número: | 1 |
| Paginació: | 115-122 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Non-alcoholic hepatic steatosis refers to the accumulation of triglycerides in the liver in the absence of alcohol consumption. Granulocyte colony-stimulating factor (G-CSF) has been reported to be an effective treatment for a variety of liver diseases. We examined the possible therapeutic effects of G-CSF on non-alcoholic hepatic steatosis in rats. Material and methods. Thirty-week-old Otsuka Long Evans Tokushima Fatty (OLETF) rats received water containing 30% sucrose for 8 weeks to promote the development of non-alcoholic hepatic steatosis. After development of the model, the rats were injected with G-CSF (100 µg/kg/day) or saline for 5 days. Four weeks after this treatment, serum levels of glucose, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and free fatty acids (FFA) were measured. Histology was examined by hematoxylin and eosin (H-E) and periodic acid Schiff (PAS) staining, and levels of expression of hepatic lipogenic enzymes were determined by RT-PCR. Results. The G-CSF-treated rats displayed significantly fewer lipid droplets than the saline-treated rats (P < 0.01), and their levels of sterol regulatory element-binding protein (SREBP)-1c, fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) mRNAs were also lower (P < 0.01), as were their liver weight and serum levels of TG and FFA (P < 0.05). Conclusion. Our results indicate that G-CSF ameliorated non-alcoholic hepatic steatosis in the OLETF rat, and this therapeutic effect involved a reduction of SREBP-1c expression. Therefore, G-CSF deserves further study as a potential treatment for non-alcoholic hepatic steatosis |
| Disciplines | Medicina |
| Paraules clau: | Gastroenterología, Medicina experimental, Terapéutica y rehabilitación, Factor estimulante de granulocitos, Hígado graso no alcohólico, Proteínas de Unión a los Elementos reguladores de los Esteroles |
| Keyword: | Gastroenterology, Experimental medicine, Therapeutics and rehabilitation, Granulocyte stimulating factor, Non alcoholic fatty liver, Sterol regulatory element-binding protein |
| Text complet: | Texto completo (Ver PDF) |
