| Revista: | Annals of hepatology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000411761 |
| ISSN: | 1665-2681 |
| Autors: | Torres Villalobos, Gonzalo1 Hamdan Pérez, Nashla1 Tovar, Armando R2 Ordaz Nava, Guillermo2 Martínez Benítez, Braulio3 Torre Villalvazo, Iván2 Morán Ramos, Sofía2 Díaz Villaseñor, Andrea2 Noriega, Lilia G2 Hiriart, Marcia4 Medina Santillán, Roberto5 Castillo Hernández, María del Carmen5 Méndez Sánchez, Nahum6 Uribe, Misael6 Torres, Nimbe2 nimbet@quetzal.innsz.mx |
| Institucions: | 1Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Cirugía Experimental, México, Distrito Federal. México 2Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Fisiología de la Nutrición, México, Distrito Federal. México 3Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Patología, México, Distrito Federal. México 4Universidad Nacional Autónoma de México, Instituto de Fisiología Celular, México, Distrito Federal. México 5Instituto Politécnico Nacional, Escuela Superior de Medicina, México, Distrito Federal. México 6Fundación Clínica Médica Sur, Unidad de Investigación de Hígado, México, Distrito Federal. México |
| Any: | 2015 |
| Període: | Jul-Ago |
| Volum: | 14 |
| Número: | 4 |
| Paginació: | 540-546 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | The study of NAFLD in humans has several limitations. Using murine models helps to understand disease pathogenesis. Aim. Evaluate the impact of 4 different diets in the production of NAFLD with emphasis on a combined high-fat plus sustained high sucrose consumption. Material and methods. Eight week-old male Wistar rats were divided in four groups and fed for 90 days with the following diets: 1) Control chow diet (C); 2) High-fat cholesterol diet (HFC) + 5% sucrose in drinking water. 3) High-fat cornstarch diet (HFCO) + 5% sucrose in drinking water. 4) Chow diet + 20% sucrose in drinking water (HSD). Metabolic changes, leptin levels, liver histology, hepatic and plasma lipid composition, fasting plasma glucose and insulin and liver gene expression of FAS, SREBP-1 and PPAR-α were evaluated. Results. The HFC diet had the highest grade of steatosis (grade 2 of 3) and HSD showed also steatosis (grade 1). Liver weight TG and cholesterol concentrations in liver were greater in the HFC diet. There were no increased levels of iron in the liver. Rats in HFC gained significantly more weight (P < 0.001). All experimental groups showed fasting hyperglycemia. HFC had the highest glucose level (158.5 ± 7 mg/dL) (P < 0.005). The HSD and the HFCO diets developed also hyperglycemia. HSD had significantly higher fasting hyperinsulinemia. Serum leptin was higher in the HFC diet (p = 0.001). In conclusion, the HFC diet with combination of high fat and high sucrose is more effective in producing NAFLD compared with a high sucrose diet only |
| Disciplines | Medicina |
| Paraules clau: | Gastroenterología, Medicina experimental, Experimentos de alimentación, Obesidad, Modelos animales, Hígado graso no alcohólico |
| Keyword: | Medicine, Experimental medicine, Gastroenterology, Feeding experiments, Obesity, Animal models, Non alcoholic fatty liver |
| Text complet: | Texto completo (Ver PDF) |
