| Revista: | Anais da Academia Brasileira de Ciencias |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000436189 |
| ISSN: | 0001-3765 |
| Autores: | Cárdenas, Denny M1 Jaimes, Miguel A1 Vega, Leidy D1 Oliveros, Nicolás L1 Soto, Javier A1 Chía, Claudia R1 Osorio, Jorge E2 Ciuoderis, Karl A3 |
| Instituciones: | 1Universidad de Santander, Facultad de Ciencias de la Salud, Cúcuta, Norte de Santander. Colombia 2University of Wisconsin, Department of Pathobiological Sciences, Madison, Wisconsin. Estados Unidos de América 3Universidad Nacional de Colombia, Centro de Investigación y Vigilancia de Enfermedades Tropicales e Infecciosas, Medellín, Antioquia. Colombia |
| Año: | 2020 |
| Volumen: | 92 |
| Número: | 1 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Zika virus appeared in South America in 2015, generating alarm worldwide as it causes microcephaly and autoimmunity. This study aims to determine the serological footprint of the incoming epidemic in a student community and to characterize the memory functional cell response during post convalescence. In a cross-sectional study, Zika-specific IgG using LIA immunoassay was found in 328 university students (CI=95%), while in the second phase, the functional cellular memory response for IFN-γ and IL-2 was quantified using post-stimulus ELISpot with inactivated virus, starting with individuals seropositive for Zika and control individuals (seropositive only for Dengue and seronegative for Zika-Dengue). Depending on the antigen used, memory humoral response (IgG) against Zika Virus was observed in >60% of the population; seropositivity for NS1 was 21.1% higher than E antigen with high intensity. The analysis of cell functionality in 22 individuals seropositive for Zika virus revealed either IFN-γ+ or IL-2+ cells in 86.3% of cases (Th1 profile), presenting multifunctionality in 50% (11 individuals), 64% of which presented> 6 SFC/104 PBMCs (>600 SFC/106 PBMC), reflecting memory circulating cells. A good agreement (Kappa= 0.754) was observed between the coexistence of both cellular and humoral responses but not in their intensity |
| Disciplinas: | Medicina |
| Palabras clave: | Inmunología, Virus, Virus del Zika, Memoria inmune, Citocinas, Seroepidemiología |
| Keyword: | Immunology, Virus, Cytokines, Immune memory, Seroepidemiology, Zika virus |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
