Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture
Título del documento: Identification of caffeic acid and rutin by UHPLC MS/MS and antioxidant activity of Commelina erecta Lineu. in cell culture
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000436194
ISSN: 0001-3765
Autores: 1
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Instituciones: 1Universidade Federal de Sergipe, Departamento de Fisiologia, Sao Cristovao, Sergipe. Brasil
2Universidade de Sao Paulo, Departamento de Bioquimica, Sao Paulo. Brasil
Año:
Volumen: 92
Número: 1
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The Commelina erecta L. (C. erecta) also known as erva-de-santa-luzia is reported by local population to have medical properties against some pathological conditions. In this study, two extracts of C. erecta leaves (aqueous and ethanolic) were phytochemically analysed and evaluated for their in-vitro antioxidant activities by DPPH, TBARS, NO assays and cell viability assays. The ultra-high performance liquid chromatography followed by tandem mass spectrometry analysis showed the presence of rutin and caffeic acid in aqueous and ethanolic extract. The total polyphenols in aqueous and ethanolic extracts found were 142.7 ± 3.0 and 123.1 ± 5.8 μg/mL of GAE, respectively. The ethanolic extract (5 mg/mL) inhibits TBARS by 33.8%, and the aqueous extract (5 mg/mL) exhibited scavenger property against nitric oxide derivatives to an extent of 77.8%. In cell culture, both extracts improved cell survivability under H2O2 induced oxidative stress. Thus, C. erecta extract is a good candidate to become a phytotherapic medicine
Disciplinas: Química
Palabras clave: Fitoquímica,
Plantas medicinales,
Polifenoles,
Acido cafeico,
Actividad antioxidante,
Commelina erecta,
Commelinaceae
Keyword: Phytochemistry,
Medicinal plants,
Polyphenols,
Antioxidant activity,
Caffeic acid,
Commelina erecta,
Commelinaceae
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