Development of an indirect ELISA assay to evaluation of the adaptive immune response of pacu (Piaractus mesopotamicus)
Título del documento: Development of an indirect ELISA assay to evaluation of the adaptive immune response of pacu (Piaractus mesopotamicus)
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000435618
ISSN: 0001-3765
Autors: 1
2
3
3
1
Institucions: 1Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Aquicultura, Jaboticabal, Sao Paulo. Brasil
2Centro Universitario Barao de Maua, Ribeirao Preto, Sao Paulo. Brasil
3Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil
Any:
Període: Dic
Volum: 90
Número: 4
Paginació: 3327-3335
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés The pacu is one of the most important species for Brazilian fish farming and is considered emerging in the global aquaculture. Despite its importance, no effective tool for evaluation of the adaptive immune response of this species has been developed. Therefore, this study aimed the development and standardization of indirect ELISA for the measurement of pacu antigen-specific antibodies using polyclonal rabbit anti-pacu IgM used as detector antibody. For this purpose was isolated and purificated of pacu IgM using mannose-binding protein affinity chromatography and produced specific polyclonal antibodies against heavy and light chains pacu IgM, that showed a molecular weight of 72 kDa and 26 kDa, respectively. Polyclonal antibodies obtained demonstrated specificity with heavy and light Ig chains of pacu serum in western blotting. These polyclonal antibodies allowed the development of an indirect ELISA assay of high sensitivity and specificity for the detection and quantification of pacu IgM antibodies immunized with bovine IgG. In conclusion, this approach has great potential to improve the monitoring of vaccine-induced immune responses and help develop immunodiagnostic and epidemiological studies of infectious diseases in pacu systems
Disciplines Medicina veterinaria y zootecnia,
Biología
Paraules clau: Peces,
Zootecnia,
Inmunología,
Peces de agua dulce,
Respuesta inmune,
ELISA,
Antisueros policlonales,
Piaractus mesopotamicus
Keyword: Fish,
Animal husbandry,
Immunology,
Freshwater fish,
Immune response,
ELISA,
Polyclonal antisera,
Piaractus mesopotamicus
Text complet: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703327&lng=en&nrm=iso&tlng=en