| Revista: | Acta scientiarum. Biological sciences |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000433515 |
| ISSN: | 1679-9283 |
| Autors: | Rodrigues, Isabela da Silva Vasconcelos1 Silva, Carolina Góes Silva e1 Silva, Rafael Salomão da1 Dolabella, Silvio Santana2 Fernandes, Marcelo Ferreira3 Fernandes, Roberta Pereira Miranda1 |
| Institucions: | 1Universidade Federal de Sergipe, Departamento de Fisiologia, Sao Cristovao, Sergipe. Brasil 2Universidade Federal de Sergipe, Departamento de Morfologia, Sao Cristovao, Sergipe. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Aracaju, Sergipe. Brasil |
| Any: | 2019 |
| Volum: | 41 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | In this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper industry. This isolate can grow and produce xylanase in medium containing wheat fiber as a substrate. The CPE of this isolate was used in preliminary testing on cellulose pulp bleaching; enzyme treatment of the pulp resulted in a 5% increase of whiteness |
| Disciplines | Biología |
| Paraules clau: | Bacterias, Biotecnología, Bacterias del suelo, Enzimas termoestables, Xilanasas, Caracterización bioquímica, Blanqueamiento, Pulpa celulósica, Bacillus |
| Keyword: | Bacteria, Biotechnology, Soil bacteria, Thermostable enzymes, Xylanases, Biochemical characterization, Bleaching, Cellulose pulp, Bacillus |
| Text complet: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/pdf |
