Development and characterization of microsatellite loci for Campomanesia xanthocarpa (Myrtaceae) and cross amplification in related species



Título del documento: Development and characterization of microsatellite loci for Campomanesia xanthocarpa (Myrtaceae) and cross amplification in related species
Revista: Acta scientiarum. Biological sciences
Base de datos: PERIÓDICA
Número de sistema: 000433599
ISSN: 1679-9283
Autors: 1
2
3
3
4
1
Institucions: 1Universidade Estadual de Londrina, Departamento de Agronomia, Londrina, Parana. Brasil
2Faculdade de Apucarana, Apucarana, Parana. Brasil
3Universidade Estadual de Londrina, Departamento de Biologia Geral, Londrina, Parana. Brasil
4Universidade Estadual de Londrina, Departamento de Ciencia Animal, Londrina, Parana. Brasil
Any:
Volum: 41
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Campomanesia xanthocarpa is a native tree, of common occurrence in almost all Brazilian Forest formations, which has its fruits and timber with high commercial value. Using an enriched genomic library we isolated and characterized microsatellite loci for C. xanthocarpa (Myrtaceae), in order to estimate genetic diversity parameters for this and related species. Twenty-eight microsatellite loci were identified and ten of them successfully amplified and showed polymorphism in a sample of 96 individuals, from four natural populations. The number of alleles per locus ranged from two to eight, and the observed and expected heterozygosities varied from 0.042 to 1.000 and from 0.294 to 0.855, respectively. These markers were tested and validated in two related species (C. eugenioides and C. guazumifolia). The microsatellite markers will be used in further studies of population genetics of C. xanthocarpa, in order to understand the genetic variability and to define the strategies needed for the conservation of the species
Disciplines Biología
Paraules clau: Genética,
Angiospermas,
Diversidad genética,
Amplificación cruzada,
Microsatélites,
Marcadores moleculares,
Campomanesia xanthocarpa,
Myrtaceae
Keyword: Genetics,
Angiosperms,
Genetic diversity,
Cross amplification,
Microsatellites,
Molecular markers,
Campomanesia xanthocarpa,
Myrtaceae
Text complet: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43454/pdf