| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000330036 |
| ISSN: | 0717-3458 |
| Autores: | Santander, Javier1 Robeson, James2 |
| Instituciones: | 1Arizona State University, Tempe, Arizona. Estados Unidos de América 2Pontificia Universidad Católica de Valparaíso, Instituto de Biología, Valparaíso. Chile |
| Año: | 2007 |
| Periodo: | Oct |
| Volumen: | 10 |
| Número: | 4 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica serovar Enteritidis (hereafter S. Enteritidis) were avirulent in the Caenorhabditis elegans animal model. We isolated phage-resistant strains of S. Enteritidis ATCC 13076 by using three lytic phages (f2αSE, f3αSE and f18αSE). In these mutants, we explored different virulence factors like lipopolysaccharide (LPS), virulence plasmid (Pla), motility and type I fimbriae, all of which may have effects on virulence and could furthermore be related to phage resistance. The phage-resistant strains of S. Enteritidis showed loss of O-Polysaccharide (O-PS) and auto-agglutination, present a rough phenotype and consequently they are avirulent in the C. elegans animal model. We speculate that the O-PS is necessary for phage attachment to the S. Enteritidis cell surface |
| Disciplinas: | Medicina |
| Palabras clave: | Terapéutica y rehabilitación, Parasitología, Salmonella enterica, Bacteriófagos, Modelos biológicos, Caenorhabditis elegans, Resistencia |
| Keyword: | Medicine, Therapeutics and rehabilitation, Parasitology, Salmonella enterica, Bacteriophages, Biological models, Caenorhabditis elegans, Resistance |
| Texto completo: | Texto completo (Ver HTML) |
