| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000413854 |
| ISSN: | 0074-0276 |
| Authors: | Sutter, Amanda1 Antunes, Deborah1 Silva-Almeida, Mariana2 Costa, Mauricio Garcia de Souza1 Caffarena, Ernesto Raul1 |
| Institutions: | 1Instituto Oswaldo Cruz, Programa de Computacao Cientifica, Rio de Janeiro. Brasil 2Instituto Oswaldo Cruz, Laboratorio de Biologia Molecular e Doencas Endemicas, Rio de Janeiro. Brasil |
| Year: | 2017 |
| Season: | Sep |
| Volumen: | 112 |
| Number: | 9 |
| Pages: | 617-625 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Leishmanolysins have been described as important parasite virulence factors because of their roles in the infection of promastigotes and resistance to host’s defenses. Leishmania (Viannia) braziliensis contains several leishmanolysin genes in its genome, especially in chromosome 10. However, the functional impact of such diversity is not understood, but may be attributed partially to the lack of structural data for proteins from this parasite. OBJECTIVES This works aims to compare leishmanolysin sequences from L. (V.) braziliensis and to understand how the diversity impacts in their structural and dynamic features. METHODS Leishmanolysin sequences were retrieved from GeneDB. Subsequently, 3D models were built using comparative modeling methods and their dynamical behavior was studied using molecular dynamic simulations. FINDINGS We identified three subgroups of leishmanolysins according to sequence variations. These differences directly affect the electrostatic properties of leishmanolysins and the geometry of their active sites. We identified two levels of structural heterogeneity that might be related to the ability of promastigotes to interact with a broad range of substrates. MAIN CONCLUSION Altogether, the structural plasticity of leishmanolysins may constitute an important evolutionary adaptation rarely explored when considering the virulence of L. (V.) braziliensis parasites |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Bioquímica, Genética, Leishmaniasis, Lisina, Metaloproteasas, Secuencia génica, Dinámica molecular, Leishmania braziliensis |
| Keyword: | Medicine, Parasitology, Biochemistry, Genetics, Leishmaniasis, Lysine, Metalloproteases, Gene sequence, Molecular dynamics, Leishmania braziliensis |
| Full text: | Texto completo (Ver HTML) |
