| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000446390 |
| ISSN: | 0074-0276 |
| Authors: | Ta Tang, Thuy-Huong1 Berzosa, Pedro1 Rubio, José Miguel2 Romay Barja, María1 Ncogo, Policarpo3 Agudo, Diego5 Herrador, Zaida1 Cerrada Gálvez, Laura5 Benito, Agustín1 |
| Institutions: | 1Instituto de Salud Carlos III, Centro Nacional de Medicina Tropical, Madrid. España 2Instituto de Salud Carlos III, Centro Nacional de Microbiología, Madrid. España 3Ministerio de Salud, Malabo. Guinea Ecutorial 4Fundación Estatal Salud, Infancia y Bienestar Social, Madrid. España 5Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, Madrid. España 6Red de Investigación Colaborativa en Enfermedades Tropicales, Madrid. España |
| Year: | 2021 |
| Volumen: | 116 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | BACKGROUND Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy is the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach for identification of L. loa filarial parasites. OBJECTIVES The aim of this study was to evaluate a Loa-Loop-mediated isothermal amplification (LAMP) assay to diagnose loiasis disease on dried blood spots (DBS) samples, compared to microscopy, filaria-real time-polymerase chain reaction (PCR) and nested-Loa PCR. METHODS A total of 100 DBS samples and 100 blood smears were used for this study. DNA was extracted using saponin/Chelex method. DNA isolated was assayed by a Loa-LAMP assay in parallel to microscopy, filaria-real time PCR and nested-Loa PCR. The sensitivities and specificities of Loa-LAMP assay was computed comparing to each one of the reference methods. FINDINGS Loa-LAMP’s sensitivity was more than 90% and specificity was nearly 100% when compared to molecular methods. On the other hand, sensitivity was decreased a bit when Loa-LAMP faced microscopy, but keeping the other statistical values high. MAIN CONCLUSIONS Loa-LAMP is an appropriate method for loiasis diagnosis in endemic areas. Though, it has disadvantages like the reagents’ high price at the moment and not to be able to detect more filarial species at once |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Diagnóstico, Loiasis, Técnicas de diagnóstico, Amplificación isotérmica, Reacción en cadena de la polimerasa (PCR), Estudios comparativos, Loa loa, Filarioidea |
| Keyword: | Parasitology, Diagnosis, Loiasis, Diagnostic techniques, Isothermal amplification, Polymerase chain reaction (PCR), Comparative studies, Loa loa, Filaroidea |
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