| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000437129 |
| ISSN: | 0074-0276 |
| Authors: | Cardoso, Thaís Cunha de Sousa1 Araújo, Carlos Bruno de1 Portilho, Laysa Gomes1 Mendes, Luiz Guilherme Alves1 Alves, Tamires Caixeta1 Silva, Gustavo Caetano1 Ribeiro, Thales Henrique Cherubino2 Gandolfi, Peterson Elizandro1 Morais, Enyara Rezende1 Amaral, Laurence Rodrigues do1 Gomes, Matheus de Souza1 |
| Institutions: | 1Universidade Federal de Uberlandia, Laboratorio de Bioinformatica e Analises Moleculares, Patos de Minas, Minas Gerais. Brasil 2Universidade Federal de Lavras, Departamento de Biologia, Lavras, Minas Gerais. Brasil |
| Year: | 2020 |
| Volumen: | 115 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Key genes control the infectivity of the Schistosoma haematobium causing schistosomiasis. A method for understanding the regulation of these genes might help in developing new disease strategies to control schistosomiasis, such as the silencing mediated by microRNAs (miRNAs). The miRNAs have been studied in schistosome species and they play important roles in the post-transcriptional regulation of genes, and in parasite-host interactions. However, genome-wide identification and characterisation of novel miRNAs and their pathway genes and their gene expression have not been explored deeply in the genome and transcriptome of S. haematobium. Identify and characterise mature and precursor miRNAs and their pathway genes in the S. haematobium genome. Computational prediction and characterisation of miRNAs and genes involved in miRNA pathway from S. haematobium genome on SchistoDB. Conserved domain analysis was performed using PFAM and CDD databases. A robust algorithm was applied to identify mature miRNAs and their precursors. The characterisation of the precursor miRNAs was performed using RNAfold, RNAalifold and Perl scripts. FINDINGS We identified and characterised 14 putative proteins involved in miRNA pathway including ARGONAUTE and DICER in S. haematobium. Besides that, 149 mature miRNAs and 131 precursor miRNAs were identified in the genome including novel miRNAs, miRNA pathway occurs in the S. haematobium, including endogenous miRNAs and miRNA pathway components, suggesting a role of this type of non-coding RNAs in gene regulation in the parasite. The results found in this work will open up a new avenue for studying miRNAs in the S. haematobium biology in helping to understand the mechanism of gene silencing in the human parasite Schistosome |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Genética, Bioinformática, MicroARNs, Expresión génica, Esquistosomiasis, Schistosoma haematobium |
| Keyword: | Parasitology, Genetics, Bioinformatics, MicroRNAs, Gene expression, Schistosomiasis, Schistosoma haematobium |
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