Journal: | Memorias do Instituto Oswaldo Cruz |
Database: | PERIÓDICA |
System number: | 000413836 |
ISSN: | 0074-0276 |
Authors: | Ramos, Bruna Alves1 Chiang, Jannifer Oliveira2 Martins, Lívia Carício2 Chagas, Liliane Leal das2 Silva, Franko de Arruda e2 Ferreira, Milene Silveira2 Freitas, Maria Nazare Oliveira2 Alcantara, Bianca Nascimento de2 Silva, Sandro Patroca da2 Miranda, Stefania Araujo3 Sepulvreda, Barbara Alves1 Correa, Layna Thayssa Guimaraes4 Negrao, Andrea Maria Goes4 Vasconcelos, Pedro Fernando da Costa2 Casseb, Alexandre do Rosario4 |
Institutions: | 1Universidade Federal do Para, Instituto de Ciencias Biologicas, Belem, Para. Brasil 2Instituto Evandro Chagas, Setor de Arbovirologia e Febres Hemorragicas, Ananindeua, Para. Brasil 3Parque Naturalistico Mangal das Garcas, Setor Veterinario, Belem, Para. Brasil 4Universidade Federal Rural da Amazonia, Instituto da Saude e Producao Animal, Belem, Para. Brasil |
Year: | 2017 |
Season: | Ago |
Volumen: | 112 |
Number: | 8 |
Pages: | 532-536 |
Country: | Brasil |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, aplicado |
English abstract | In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses. OBJECTIVES This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses. METHODS Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled. Upon capture, the birds were subjected to a clinical examination in search of alterations that could indicate the presence of arboviruses. Blood samples were converted to serum and tested using the haemagglutination inhibition (HI) technique with a panel of 19 antigens of arboviruses circulating in the Amazon. The confirmation assay for the positive reactions to the viral species tested by HI was a neutralisation test in new-born Swiss albino mice (Mus musculus) [mouse neutralisation test (MNT)]. FINDINGS A total of 10 (11.8%) serum samples tested positive for antiflavivirus antibodies by HI. All the samples positive for the HI test were subjected to MNT for detection of viruses and yielded negative results (logarithmic neutralisation index < 1.7). MAIN CONCLUSION The results represent the first serological detection of antiarbovirus antibodies in domestic pigeons as potential hosts of arboviruses in Brazil. The detection of haemagglutination-inhibiting antibodies against genus Flavivirus indicated that there was recent contact between the analysed domestic pigeons and these arboviruses. Further studies are needed to evaluate the role of free-living pigeons in the maintenance cycle and spread of arboviruses in the Amazon |
Disciplines: | Medicina |
Keyword: | Microbiología, Virus, Aves, Arbovirus, Mecanismos de infección, Reservorios animales, Pichones, Pruebas serológicas |
Keyword: | Medicine, Microbiology, Virus, Birds, Arbovirus, Infection mechanisms, Animal reservoirs, Pigeons, Serological tests |
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