| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000437111 |
| ISSN: | 0074-0276 |
| Authors: | Boucinha, Carolina1 Caetano, Amanda R1 Santos, Helena L.C1 Helaers, Raphael2 Vikkula, Miikka2 Branquinha, Marta Helena3 Santos, André Luis Souza dos3 Grellier, Philippe4 Morelli, Karina Alessandra1 d‘Avila Levy, Claudia Masini1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 2Universite de Louvain, de Duve Institute, Bruselas. Bélgica 3Universidade Federal do Rio de Janeiro, Instituto de Microbiologia "Paulo de Goes", Rio de Janeiro. Brasil 4Museum National d'Histoire Naturelle, Unite Molecules de Communication et Adaptation des Microorganisme, Paris. Francia |
| Year: | 2020 |
| Volumen: | 115 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | BACKGROUND Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity. METHODS We sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br. OBJECTIVE The main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidae genera, to generate phylogenetic trees that can simplify new isolates screenings. FINDINGS A DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits. MAIN CONCLUSIONS This survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections |
| Disciplines: | Biología |
| Keyword: | Protozoarios, Taxonomía y sistemática, Clasificación taxonómica, Código de barras, Cinetoplastos, Trypanosomatidae |
| Keyword: | Protozoa, Taxonomy and systematics, Taxonomic classification, Barcodes, Kinetoplasts, Trypanosomatidae |
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