| Journal: | Acta bioquímica clínica latinoamericana |
| Database: | PERIÓDICA |
| System number: | 000365225 |
| ISSN: | 0325-2957 |
| Authors: | Facio, María Laura1 Madalena, Leticia1 Fraind, Susana1 Alejandre, Mariel1 Bresciani, Pablo1 Pizzolato, Marco1 |
| Institutions: | 1Universidad de Buenos Aires, Departamento de Bioquímica Clínica, Buenos Aires. Argentina |
| Year: | 2013 |
| Season: | Mar |
| Volumen: | 47 |
| Number: | 1 |
| Pages: | 37-46 |
| Country: | Argentina |
| Language: | Español |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| Spanish abstract | En este trabajo se presenta un método confiable para el análisis de rutina de proteínas urinarias. El método es la electroforesis bidimensional que combina la electroforesis en acetato de celulosa con la electroforesis en gel de poliacrilamida con dodecil sulfato de sodio seguida de tinción argéntica. La electroforesis bidimensional abre el espectro de proteínas, siendo algunas de ellas identificadas por immunoblotting o espectrometría de masa MALDI. Dichas proteínas son de bajo peso molecular: Orosomucoide (40 kDa), apolipoproteína AI (28 kDa), zinc-alfa 2-glicoproteína (43 kDa), fragmento del perlecan C-terminal LG3 (23 kDa), prostaglandina D2 sintasa tipo-lipocalina (29 kDa) e inter-alfa inhibidor de tripsina cadena pesada H4 (35 kDa). Estas proteínas están incluidas en estudios de falla renal aguda, falla renal crónica, trasplante renal, enfermedad glomerular y enfermedad maligna del tracto urogenital. El método, no invasivo, se aproxima a la tecnología emergente de la proteómica que permite el análisis simultáneo de varias proteínas urinarias como una herramienta para el diagnóstico y monitoreo de una variedad de enfermedades humanas |
| English abstract | A method suitable for routine clinical analyses of urinary proteins is presented. This method is a two-dimensional electrophoresis procedure, combining cellulose acetate electrophoresis and sodium dodecyl sulfate-polyacrylamide gels electrophoresis followed by silver staining. The resulting two-dimensional electrophoresis opened the protein spectrum and some of the latter were identified, by immunoblotting or MALDI mass spectrometry. There are low molecular weight protein: Orosomucoid (40 kDa), apolipoprotein AI (28 kDa), Zinc-alpha2-glycoprotein (43 kDa), C-terminal perlecan fragment LG3 (23 kDa), lipocalin-type prostaglandin D2 synthase (29 kDa) and inter-alpha-trypsin inhibitor heavy chain H4 (35 kDa). They include studies of acute and chronic kidney injury, renal transplantation, glomerular disease and malignancy of the urogenital tract. This method approaching the emerging proteomic technologies allows simultaneous examination of the patterns of multiple urinary proteins as a powerful non-invasive tool for diagnosis and monitoring of variety of human diseases |
| Portuguese abstract | electrophoresis opened the protein spectrum and some of the latter were identified, by immunoblotting or MALDI mass spectrometry. There are low molecular weight protein: Orosomucoid (40 kDa), apolipoprotein AI (28 kDa), Zinc-alpha2-glycoprotein (43 kDa), C-terminal perlecan fragment LG3 (23 kDa), lipocalin-type prostaglandin D2 synthase (29 kDa) and inter-alpha-trypsin inhibitor heavy chain H4 (35 kDa). They include studies of acute and chronic kidney injury, renal transplantation, glomerular disease and malignancy of the urogenital tract. This method approaching the emerging proteomic technologies allows simultaneous examination of the patterns of multiple urinary proteins as a powerful non-invasive tool for diagnosis and monitoring of variety of human diseases |
| Disciplines: | Química, Medicina |
| Keyword: | Bioquímica, Diagnóstico, Nefrología, Electroforesis bidimensional, Orina, Zinc-alfa2-glicoproteína, Péptido LG3, Prostaglandinas, Inter-alfa-tripsina inhibidor H4, Endotelio vascular, Bioquímica clínica |
| Keyword: | Chemistry, Medicine, Biochemistry, Diagnosis, Nephrology, Two-dimensional electrophoresis, Urine, Zinc-alpha2-glycoprotein, Fragment LG3, Prostaglandin D2 synthase, Inter-alpha-trypsin inhibitor H4, Vascular endothelial, Clinical biochemistry |
| Full text: | Texto completo (Ver PDF) |
