| Journal: | The journal of venomous animals and toxins including tropical diseases |
| Database: | PERIÓDICA |
| System number: | 000298946 |
| ISSN: | 1678-9199 |
| Authors: | Lee, K.H1 Won, H.J Kim, S.N Yoo, S.H Shin, I.S Shin, K.H Hong, S.H Lee, S. H2 Min, H.K Park, S.N Hur, S.J |
| Institutions: | 1Korea Food and Drug Administration, Department of Biologics Evaluation, Seúl. Corea del Sur 2National Institute of Toxicological Research, Korea Food and Drug Administration, Seúl. Corea del Sur |
| Year: | 2006 |
| Volumen: | 12 |
| Number: | 4 |
| Pages: | 560-577 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | In Korea, antivenoms for the treatment of patients bitten by venomous snakes have been imported from Japan or China. Although there is cross-reactivity between these antibodies and venoms from snakes indigenous to Korea (e.g. Agkistrodon genus), protection is not optimal. Antivenoms specifically prepared to neutralize Korean snake venoms could be more effective, with fewer side effects. To this end, we established an infrastructure to develop national standards and created a standardized method to evaluate the efficacy of two horse-derived antivenoms using mouse lethal toxin test. Additionally, we determined the antivenoms neutralizing activity against lethal doses (LD50) of Agkistrodon halys (from Japan) and Jiangzhe Agkistrodon halys (from China) venoms. We also performed cross-neutralization tests using probit analysis on each pairing of venom and antivenom in order to check the possibility of using Jiangzhe A. halys venom as a substitute for A. halys venom, the current standard. Slope of A. halys venom with A. halys antivenom was 10.2 and that of A. halys venom with Jiangzhe A. halys antivenom was 9.6. However, Slope of Jiangzhe A. halys venom with A. halys antivenom was 4.7 while that of Jiangzhe A. halys venom with Jiangzhe A. halys antivenom was 11.5. Therefore, the significant difference in slope patterns suggests that Jiangzhe A. halys venom cannot be used as a substitute for the standard venom to test the anti-lethal toxin activity of antivenoms (p<0.05) |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Toxicología, Veneno de víbora, Actividad letal, Prueba de potencia, Toxina anti-letal, Análisis probit, Agkistrodon halys, Viperidae |
| Keyword: | Medicine, Pharmacology, Toxicology, Snake venom, Lethal activity, Potency test, Anti-lethal toxin, Probit analysis, Agkistrodon halys, Viperidae |
| Full text: | Texto completo (Ver HTML) |
