| Journal: | The journal of venomous animals and toxins including tropical diseases |
| Database: | PERIÓDICA |
| System number: | 000299001 |
| ISSN: | 1678-9199 |
| Authors: | Tamieti, B.P1 Damatta, R.A2 Cogo, J.C Silva, N.S. da Mittmann, J Pacheco-Soares, C |
| Institutions: | 1Universidade do Vale do Paraiba, Instituto de Pesquisa e Desenvolvimento, Sao Jose dos Campos, Sao Paulo. Brasil 2Universidade Estadual do Norte Fluminense, Centro de Biociencias e Biotecnologia, Campos dos Goytacazes, Rio de Janeiro. Brasil |
| Year: | 2007 |
| Volumen: | 13 |
| Number: | 1 |
| Pages: | 56-68 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis |
| Disciplines: | Biología |
| Keyword: | Biología celular, Toxicología, Veneno de víbora, Citoesqueleto, Retículo endoplásmico, Apoptosis, Crotalus durissus terrificus, Viperidae |
| Keyword: | Biology, Cell biology, Toxicology, Snake venom, Cytoskeleton, Endoplasmic reticulum, Apoptosis, Crotalus durissus terrificus, Viperidae |
| Full text: | Texto completo (Ver HTML) |
