| Journal: | Revista mexicana de fitopatología |
| Database: | PERIÓDICA |
| System number: | 000408871 |
| ISSN: | 0185-3309 |
| Authors: | Avila Alistac, Norma1 Ramírez Rojas, Sergio2 Lozoya Saldaña, Héctor3 Rebollar Alviter, Angel4 Guzmán Plazola, Remigio Anastacio1 |
| Institutions: | 1Colegio de Postgraduados, Campus Montecillo, Montecillo, Estado de México. México 2Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Zacatepec, Zacatepec, Morelos. México 3Universidad Autónoma Chapingo, Departamento de Horticultura, Chapingo, Estado de México. México 4Universidad Autónoma Chapingo, Centro Regional Morelia, Morelia, Michoacán. México |
| Year: | 2017 |
| Season: | May |
| Volumen: | 35 |
| Number: | 2 |
| Pages: | 242-262 |
| Country: | México |
| Language: | Español, inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| Spanish abstract | El objetivo de la investigación fue identificar hospedantes alternos de Iris yellow spot virus (IYSV) y establecer el rango de hospedantes del putativo vector(es) en regiones productoras de cebolla (Allium cepa) de Morelos y Michoacán, México. En 10 localidades de ambos estados se muestrearon cultivos de cebolla y arvenses, en presencia y ausencia del cultivo. Las plantas de cebolla se analizaron por RT-PCR y las arvenses por DAS-ELISA. Las arvenses se identificaron a nivel especie, los trips colectados de las mismas se establecieron colonias para su identificación por PCR, con iniciadores específicos que amplifican un segmento del gen de citocromo oxidasa I (COI). Se analizaron e identificaron 295 arvenses agrupadas en 56 especies (23 familias), todas resultaron negativas para IYSV. Se detectaron trips en 75 arvenses agrupadas en 17 especies. Se analizaron 33 poblaciones de trips (22 de Morelos y 11 de Michoacán). La secuenciación indicó identidad con Thrips tabaci con una homología superior a 97 %. Las arvenses Ricinus communis y Acalypha ostryifolia registraron el mayor número de T. tabaci. En cebolla se confirmó la presencia de IYSV con RT-PCR en las 10 parcelas muestreadas. Este es el primer reporte de la presencia de IYSV en el estado de Michoacán |
| English abstract | The main goal of this research was to identify alternate hosts of Iris yellow spot virus (IYSV) and to establish the range of hosts of the putative vector(s) in onion (Allium cepa) producing regions of Morelos and Michoacan, Mexico. In 10 localities of both states, onion crops and weeds were sampled in the presence and absence of the crop. Onion plants were analyzed by RT-PCR and the weeds by DAS-ELISA. The weeds were identified at species level, the thrips collected from weeds were established colony for identification by PCR, with specific primers that amplify a segment of the cytochrome oxidase I (COI) gene. A total of 295 weeds grouped in 56 species (23 families) were analyzed and identified. All the weeds were negative for IYSV. Thrips were detected on 75 weeds grouped in 17 species. Thirty-three thrips populations were analyzed (22 from Morelos and 11 from Michoacan). Sequencing indicated identity with Thrips tabaci with a homology greater than 97 %. Weeds Ricinus communis and Acalypha ostryifolia were found with high number of T. tabaci. In onion, the presence of IYSV was confirmed with RT-PCR in the ten field plots sampled. This is the first report of IYSV in Michoacan state |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Hortalizas, Insectos, Virus, Tospovirus, Iris yellow spot virus, Trips, Thrips tabaci, Cebolla, Ricinus communis, Acalypha ostryifolia, Plantas arvenses, Michoacán, Morelos, México, PCR, COI |
| Keyword: | Agricultural sciences, Biology, Phytopathology, Vegetables, Weeds, Insects, Virus, Tospovirus, Iris yellow spot virus, Trips, Thrips tabaci, Onion, Ricinus communis, Acalypha ostryifolia, Michoacan, Morelos, Mexico, PCR, COI |
| Full text: | Texto completo (Ver HTML) |
