| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000405545 |
| ISSN: | 0074-0276 |
| Authors: | Yamashiro, Sandra1 Fiuza, Vagner Ricardo da Silva2 Teixeira, Angela Therezinha Lauand Sampaio3 Branco, Nilson1 Levy, Carlos Emilio3 Castro, Isabel Cristina Vidal Siqueira de1 Franco, Regina Maura Bueno1 |
| Institutions: | 1Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil 2Universidade Estadual de Campinas, Faculdade de Engenharia Civil, Arquitetura e Urbanismo, Campinas, Sao Paulo. Brasil 3Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil |
| Year: | 2017 |
| Season: | Jun |
| Volumen: | 112 |
| Number: | 6 |
| Pages: | 404-410 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spores, but can also result from direct contact with spores through broken skin, eye lesions, and sexual transmission, depending on the microsporidian species. Although there are reports of E. bieneusi found in humans and animals in Brazil, there are no published studies of environmental samples examined by molecular methods. OBJECTIVES The purpose of this study was to verify the presence of E. bieneusi in raw sewage and treated effluent from a combined system by molecular methods. METHODS Raw sewage and treated effluent samples collected from a combined system were analysed for the presence of E. bieneusi using the internal transcriber spacer (ITS) region of E. bieneusi by nested polymerase chain reaction. FINDINGS The analysis revealed E. bieneusi presence and a novel genotype (EbRB) in one raw sewage sample and one treated effluent. MAIN CONCLUSIONS The presence of E. bieneusi in final effluent indicates that the combined system may not remove microsporidian spores. This study is the first report of E. bieneusi in environmental samples in Brazil |
| Disciplines: | Medicina |
| Keyword: | Microbiología, Salud pública, Aguas residuales, Efluentes urbanos, Tratamiento de aguas, Monitoreo microbiológico, Enterocytozoon bieneusi, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Medicine, Microbiology, Public health, Sewage, Urban effluents, Waste water treatment, Microbiological screening, Enterocytozoon bieneusi, Polymerase chain reaction (PCR) |
| Full text: | Texto completo (Ver HTML) |
