| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000408515 |
| ISSN: | 1415-4757 |
| Authors: | Santos, Tiago Benedito dos1 Lima, Joni Esrom2 Felicio, Mariane Silva1 Soares, Joao Danillo Moura1 Domingues, Douglas Silva1 |
| Institutions: | 1Instituto Agronomico do Parana, Laboratorio de Biotecnologia Vegetal, Londrina, Parana. Brasil 2Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil |
| Year: | 2017 |
| Volumen: | 40 |
| Pages: | 346-359 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Nitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3–) and ammonium (NH4+) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome-wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome. We analyzed the expression of six selected transporters in Coffea arabica roots submitted to N deficiency. N source preference was also analyzed in C. arabica using isotopes. C. canephora N transporters follow the patterns observed for most eudicots, where each member of the AMT and NRT families has a particular role in N mobilization, and where some of these are modulated by N deficiency. Despite the prevalence of putative nitrate transporters in the Coffea genome, ammonium was the preferential inorganic N source for N-starved C. arabica roots. This data provides an important basis for fundamental and applied studies to depict molecular mechanisms involved in N uptake in coffee trees |
| Disciplines: | Biología, Agrociencias |
| Keyword: | Fisiología vegetal, Frutales, Café, Nutrición mineral, Transporte de nutrientes, Nitrógeno, Regulación génica, Coffea canephora, Coffea arabica, Rubiaceae |
| Keyword: | Biology, Agricultural sciences, Plant physiology, Fruit trees, Coffee, Mineral nutrition, Nutrients transport, Nitrogen, Gene regulation, Coffea canephora, Coffea arabica, Rubiaceae |
| Full text: | Texto completo (Ver HTML) |
