| Journal: | Biotecnología vegetal |
| Database: | PERIÓDICA |
| System number: | 000346858 |
| ISSN: | 1609-1841 |
| Authors: | López Torres, Jorge1 Kosky, Rafael G2 Montaño Pérez, Nery1 Reinaldo Alvarez, Damicela1 Rayas Cabrera, Ayme1 Cabrera Jova, Manuel1 Santos Pino, Arletys1 Medero Vega, Víctor1 Basail Pérez, Milagros1 Roux, Nicolás3 |
| Institutions: | 1Instituto de Investigaciones de Viandas Tropicales, Santo Domingo, Villa Clara. Cuba 2Universidad Central "Marta Abreu" de Las Villas, Instituto de Biotecnología de las Plantas, Santa Clara, Villa Clara. Cuba 3Parc Scientifique Agropolis, Montpellier, Herault. Francia |
| Year: | 2012 |
| Season: | Ene-Mar |
| Volumen: | 12 |
| Number: | 1 |
| Pages: | 25-31 |
| Country: | Cuba |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A method has been developed for plant regeneration via embryogenic cell suspensions from diploid cultivar ‘Calcutta 4´. For callus induction with embryogenic structures, different plant tissues such as scalps from cauliflower-like meristems and meristematic domes of axillary sprouted buds in combination with several culture media were evaluated as explants. The best embryogenic response (8%) was noticed with meristematic domes of axillary sprouted buds in culture medium Murashige and Skoog salts at 50%, MS vitamins, 30 g l-1 sucrose, 10 mg l-1 ascorbic acid, 1 mg l–1 2,4-D, 0.22 mg l–1 Zeatine and supplemented with 100 mg l–1 malt extract, 100 mg l–1 glutamine, 1 mg l–1 biotin, 200 mg l–1 casein hydrolysate, 4.0 mg l–1 proline and solidified with 2.0 g l–1 Gelrite. Embryogenic cell suspensions were established and the highest increase of cellular biomass with 0.50 ml settled cell volume (SCV) was obtained 18 days after culture initiation. In a RD1 culture medium, embryogenic masses from 1362 to 2480 embryos were formed. An average of 54.5% germinated embryos in Temporal Immersion System (TIS) was obtained. Results were significantly higher in comparison with the use of semisolid culture media (28%). Regenerated plants are in field conditions to value their genetic stability |
| Disciplines: | Biología |
| Keyword: | Fisiología vegetal, Frutales, Yemas axilares, Embriogénesis somática, Plátano, Musa |
| Keyword: | Biology, Plant physiology, Fruit trees, Axillary buds, Somatic embryogenesis, Banana, Musa |
| Full text: | Texto completo (Ver PDF) |
