| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000409393 |
| ISSN: | 1665-2681 |
| Authors: | Bautista, Wendy1 Lipschitz, Jeremy2 McKay, Andrew2 Minuk, Gerald Y1 |
| Institutions: | 1University of Manitoba, Department of Medicine, Winnipeg, Manitoba. Canadá 2University of Manitoba, Department of Surgery, Winnipeg, Manitoba. Canadá |
| Year: | 2017 |
| Season: | Mar-Abr |
| Volumen: | 16 |
| Number: | 2 |
| Pages: | 297-303 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, analítico |
| English abstract | The inability to distinguish cancer (CSCs) from normal stem cells (NSCs) has hindered attempts to identify safer, more effective therapies for hepatocellular carcinoma (HCC). The aim of this study was to document and compare cell membrane potential differences (PDs) of CSCs and NSCs derived from human HCC and healthy livers respectively and determine whether altered GABAergic innervation could explain the differences. Material and methods. Epithelial cell adhesion molecule (EpCAM) positive stem cells were isolated from human liver tissues by magnetic bead separations. Cellular PDs were recorded by microelectrode impalement of freshly isolated cells. GABAA receptor subunit expression was documented by reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence. Results. Results. CSCs were significantly depolarized (-7.0 Results. ± 1.3 mV) relative to NSCs (-23.0 ± 1.4 mV, p < 0.01). The depolarized state was associated with different GABAA receptor subunit expression profiles wherein phasic transmission, represented by GAGAA α3 subunit expression, was prevalent in CSCs while tonic transmission, represented by GABAA α6 subunit expression, prevailed in NSCs. In addition, GABAA subunits α3, β3, γ3 and δ were strongly expressed in CSCs while GABAA π expression was dominant in NSCs. CSCs and NSCs responded similarly to GABAA receptor agonists (ΔPD: 12.5 ± 1.2 mV and 11.0 ± 3.5 mV respectively). Conclusion. The results of this study indicate that CSCs are significantly depolarized relative to NSCs and these differences are associated with differences in GABA |
| Disciplines: | Medicina |
| Keyword: | Diagnóstico, Gastroenterología, Oncología, Carcinoma hepatocelular, Potencial de membrana, Células troncales cancerosas, Células troncales, GABA, Hígado |
| Keyword: | Medicine, Diagnosis, Gastroenterology, Oncology, Hepatocellular carcinoma, Cancer stem cells, Stem cells, GABA, Liver |
| Full text: | Texto completo (Ver PDF) |
